Human-Induced Pluripotent Stem Cells
نویسندگان
چکیده
Human-Induced Pluripotent Stem Cells The ability to reprogram adult mammalian somatic cells to a pluripotent state has proven to be a paradigm shifting discovery. In 2007, this reprogramming technology was translated to the engineering of human-induced pluripotent stem cells (hiPSCs) using skin fibroblasts by both the Yamanaka (transduction of c-Myc, Oct3/4, SOX2, and Klf4) and Thomson (transduction of Oct3/4, SOX2, Nanog, and Lin28) laboratories. Importantly, human donors could be fully phenotyped, and their hiPSCs, which retain their genetic blueprint, could be isolated and used to study human disease mechanisms in vitro. Given the potential use of these cells in understanding and treating disease, Feaster et al, in this issue of Circulation Research, optimize the culture conditions to promote the differentiation of hiPSC-derived cardiomyocytes.
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Large-Scale Expansion of Human Embryonic and Induced Pluripotent Stem Cells for Cell Therapy Applications
Successful isolation, derivation and culturing of human pluripotent stem cells, including human embryonic stem cells (hESCs) and human induced pluripotent stem (hiPSCs) cells in laboratory scale has opened new horizones for cell therapy applications such as tissue engineering and regenerative medicine. However, most of the cell therapy protocols using these unique cells require large number of ...
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